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Journal / Cancer Research / Målet cellen pre berikelse og hele genomet…
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Cancer Research

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JoVE Journal Cancer Research
Målet cellen pre berikelse og hele genomet forsterkning for enkeltcelle nedstrøms karakteristikk
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Målet cellen pre berikelse og hele genomet forsterkning for enkeltcelle nedstrøms karakteristikk

Article DOI: 10.3791/56394-v • 10:12 min • May 15th, 2018
May 15th, 2018 •
*1, *1, 1, 2, 3, 3, 4, 1,5, 1
1Institute of Cell Biology, Histology and Embryology, Medical University of Graz, 2Institute of Pathology, Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center, University of Gothenburg
* These authors contributed equally

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Denne protokollen er å komme seg og forberede sjeldne målcellene fra en blanding med ikke-mål bakgrunn celler for molekylær genetisk karakterisering på encellede nivå. DNA kvalitet er ikke behandlet enkeltceller og tillater encellede program (begge screening basert og målrettet analyse).

Tags

Kreftforskning problemet 135 sjeldne celle analyse enkelt celle analyse hele genomet forsterkning matrise sammenlignende genomet hybridisering i vivo isolasjon apparat mål neste generasjons sekvensering neste generasjons sekvensering immunofluorescence merking
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