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Journal / Genetics / Grupperte shRNA skjermen for aktivering av MeCP2…
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JoVE Journal Genetics
Grupperte shRNA skjermen for aktivering av MeCP2 på inaktive X-kromosomet
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Grupperte shRNA skjermen for aktivering av MeCP2 på inaktive X-kromosomet

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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Vi rapporterer en liten hårnål RNA (shRNA) og neste generasjons sekvensering-basert protokoll for å identifisere regulatorer av X-chromosome inaktivering i murine celle linje med firefly luciferase og hygromycin motstand gener del av methyl CpG bindende protein 2 ( MeCP2) Gen på inaktive X-kromosomet.

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Genetikk problemet 133 X-chromosome inaktivering epigenetics MeCP2 Retts syndrom XIST shRNA screening
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