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Journal / Genetics / ShRNAs combinados pantalla para reactivación de…
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JoVE Journal Genetics
ShRNAs combinados pantalla para reactivación de MeCP2 en el cromosoma de X inactivo
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ShRNAs combinados pantalla para reactivación de MeCP2 en el cromosoma de X inactivo

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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Divulgamos una pequeña horquilla RNA (shRNA) y el siguiente protocolo basado en la secuencia de generación para la identificación de reguladores de la inactivación del cromosoma x en una línea celular murina con luciferasa de luciérnaga y genes de resistencia a Higromicina fusionado a la metil CpG Unión proteína 2 ( MeCP2) gen en el cromosoma X inactivo.

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Número 133 la inactivación del X-cromosoma genética epigenética MeCP2 Rett síndrome XIST shRNA proyección
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