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Journal / Genetics / In pool shRNA schermo per riattivazione di MeCP2…
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JoVE Journal Genetics
In pool shRNA schermo per riattivazione di MeCP2 sul cromosoma di X inattivo
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In pool shRNA schermo per riattivazione di MeCP2 sul cromosoma di X inattivo

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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Segnaliamo un short hairpin RNA (shRNA) e sequenziamento basato su protocollo di ultima generazione per l'identificazione dei regolatori di inattivazione del cromosoma x in una linea cellulare murina con luciferasi firefly e geni di resistenza igromicina fuse con il metil CpG binding protein 2 ( MeCP2) gene sul cromosoma di X inattivo.

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Problema 133 inattivazione del cromosoma x genetica epigenetica MeCP2 shRNA XIST la sindrome di Rett di screening
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