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Journal / Genetics / プールされた shRNA MeCP2 再アクティブ化非アクティブ X…
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Genetics

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JoVE Journal Genetics
プールされた shRNA MeCP2 再アクティブ化非アクティブ X 染色体上の画面
A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
Journal (Genetics)
A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
Journal (Genetics)
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
 
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プールされた shRNA MeCP2 再アクティブ化非アクティブ X 染色体上の画面

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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小さいヘアピン RNA (shRNA) とホタルのルシフェラーゼとマウス細胞における x 染色体不活性化のレギュレータを識別するの次世代シーケンス ベースのプロトコルについて報告して、メチル CpG 結合蛋白質 2 (hygromycin 抵抗性遺伝子融合MeCP2)不活性 X 染色体の遺伝子。

Tags

遺伝学、問題 133、x 染色体の不活性化、エピジェネティクス、MeCP2、レット症候群、XIST、shRNA スクリーニング
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