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JoVE Journal
Neuroscience

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血脑屏障的高分辨率共聚焦成像
 
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血脑屏障的高分辨率共聚焦成像: 成像、3D 重建和 Transcytosis 的量化

Article DOI: 10.3791/56407-v 10:30 min November 16th, 2017
November 16th, 2017

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Summary

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在这里, 我们提出了一个 microscopy-based 协议的高分辨率成像和三维重建的小鼠神经血管单位和血脑屏障使用脑漂浮部分。这种方法可用于血脑屏障内细胞器的可视化、分析和量化。

Tags

神经生物学 问题 129 血脑屏障 transcytosis 细胞内转运 共聚焦显微镜 免疫荧光 图像分析
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