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Journal / Immunology and Infection / Sovrapressione e purificazione dell'uomo
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Immunology and Infection

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JoVE Journal Immunology and Infection
Sovrapressione e purificazione dell'uomo
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Sovrapressione e purificazione dell'uomo

Article DOI: 10.3791/56430-v • 07:35 min • August 3rd, 2017
August 3rd, 2017 •
*1, *1, 2, 2, 1,3, 1,3, 2, 2,4, 2
1Sackler Faculty of Medicine, Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University
* These authors contributed equally

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Un semplice protocollo di sovraespressione e di purificazione della cis -preniltransferasi umana ottimizzata dal codone, in condizioni non denaturanti, da Escherichia Coli , è stato descritto, insieme ad un test enzimatico di attività. Questo protocollo può essere generalizzato per la produzione di altre proteine ​​di cis- preniltransferasi in quantità e qualità adatte agli studi meccanici.

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Immunologia Numero 126 ottimizzazione del codone sovraespressione eterologa purificazione della proteina preniltransferasi deidrodolichilidifosfato sintasi polisteni
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