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Journal / Immunology and Infection / 高通量并行测序在金黄叙利亚仓鼠感染期间测量钩端螺旋体螺旋体转插入突变体的适应性…
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Immunology and Infection

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JoVE Journal Immunology and Infection
高通量并行测序在金黄叙利亚仓鼠感染期间测量钩端螺旋体螺旋体转插入突变体的适应性
A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
Journal (Immunology and Infection)
A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
Antigenic Liposomes for Generation of Disease-specific Antibodies
Journal (Immunology and Infection)
Antigenic Liposomes for Generation of Disease-specific Antibodies
 
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高通量并行测序在金黄叙利亚仓鼠感染期间测量钩端螺旋体螺旋体转插入突变体的适应性

Article DOI: 10.3791/56442-v • 11:50 min • December 18th, 2017
December 18th, 2017 •
1,2, 1,2, 1,2, 1,2,3,4
1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine, David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology, David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles

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我们在这里描述的技术, 结合转诱变和高通量测序, 以确定和量化转端突变体在组织后的挑战, 仓鼠。该协议可用于筛选动物生存和传播的变种人, 也可用于体外研究。

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免疫学 问题 130 高通量排序 随机诱变 巢式 PCR 共轭,钩端螺旋体 健身 突变体 图书馆
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