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Journal / Immunology and Infection / High throughput sequenziamento parallelo al…
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Immunology and Infection

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JoVE Journal Immunology and Infection
High throughput sequenziamento parallelo al Fitness misura di Leptospira interrogans trasposone inserimento mutanti durante dorato siriano criceto infezione
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High throughput sequenziamento parallelo al Fitness misura di Leptospira interrogans trasposone inserimento mutanti durante dorato siriano criceto infezione

Article DOI: 10.3791/56442-v • 11:50 min • December 18th, 2017
December 18th, 2017 •
1,2, 1,2, 1,2, 1,2,3,4
1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine, David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology, David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles

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Qui descriviamo una tecnica che combina la mutagenesi transposon con sequenziamento ad alta produttività per identificare e quantificare il trasposone leptospirale mutanti in tessuti dopo una sfida di criceti. Questo protocollo può essere utilizzato per mutanti di schermo per la sopravvivenza e la diffusione negli animali e può essere applicato anche agli studi in vitro .

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Immunologia problema 130 High-throughput sequencing coniugazione di mutagenesi casuale PCR annidata Leptospira fitness mutanti biblioteca
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