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Journal / Immunology and Infection / Høy gjennomstrømming parallelle sekvensering å…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Høy gjennomstrømming parallelle sekvensering å måle Fitness Leptospira interrogans Transposon innsetting mutanter under Golden Syrian Hamster infeksjon
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
Journal (Immunology and Infection)
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Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Journal (Immunology and Infection)
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
 
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Høy gjennomstrømming parallelle sekvensering å måle Fitness Leptospira interrogans Transposon innsetting mutanter under Golden Syrian Hamster infeksjon

Article DOI: 10.3791/56442-v • 11:50 min • December 18th, 2017
December 18th, 2017 •
1,2, 1,2, 1,2, 1,2,3,4
1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine, David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology, David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles

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Her beskriver vi en teknikk som kombinerer transposon mutagenese med høy gjennomstrømming sekvensering å identifisere og telle transposon leptospiral mutanter i vev etter en utfordring hamstere. Denne protokollen kan brukes til skjermen mutanter for overlevelse og spredning i dyr og kan også bli brukt i vitro studier.

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Immunologi problemet 130 høy gjennomstrømming sekvensering tilfeldig mutagenese nestede PCR Bøyning Leptospira fitness mutanter bibliotek
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