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Journal
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Immunology and Infection
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替代
体外
测定病毒衣壳结构完整性的方法
JoVE Journal
Immunology and Infection
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Immunology and Infection
Alternative
In Vitro
Methods for the Determination of Viral Capsid Structural Integrity
Please note that all translations are automatically generated.
Click here for the English version.
替代
体外
测定病毒衣壳结构完整性的方法
DOI:
10.3791/56444-v
•
12:57 min
•
November 16, 2017
•
Matthew D. Moore
,
Brittany S. Mertens
,
Lee-Ann Jaykus
1
Department of Food, Bioprocessing, and Nutrition Sciences
,
North Carolina State University
,
2
Department of Chemical and Biomolecular Engineering
,
North Carolina State University
Chapters
00:05
Title
00:49
Plate-based Binding Assay for Evaluating Loss of Higher Order Norovirus Capsid Structure
04:44
Dynamic Light Scattering (DLS) Detecting Virus Aggregation After Heat Treatment
06:32
DLS for Detecting Virus Aggregation in Real Time
07:29
Transmission Electron Microscopy (TEM) Sample Preparation
09:34
Results: Reduction of Human Norovirus Capsid Stability After Heating
11:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
利用病毒基因组扩增的常规检测方法由于不能区分传染性的非传染性微粒而受到限制。本文的目的是提供的其他方法, 以帮助区分传染性病毒颗粒使用适结合, 动态光散射和透射电子显微镜详细的协议。
Tags
Viral Capsid
Structural Integrity
In Vitro Methods
Norovirus
Viral Inactivation
Capsid Protein
Heat Treatment
Aptamer
ELISA
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