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Developmental Biology

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JoVE Journal Developmental Biology
Kvantitative hele-mount immunofluorescens analyse af Cardiac stamfader populationer i mus embryoner
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Kvantitative hele-mount immunofluorescens analyse af Cardiac stamfader populationer i mus embryoner

Article DOI: 10.3791/56446-v • 09:42 min • October 12th, 2017
October 12th, 2017 •
1,2,3, 4, 5,6, 1,2,3
1Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Microscopy Core, Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

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Her, beskriver vi en protokol for hele mount immunofluorescens og image-baserede kvantitative volumetrisk analyse af tidlige fase mus embryoner. Vi præsenterer denne teknik som en kraftfuld tilgang til kvalitativt og kvantitativt vurdere hjerte strukturer under udvikling, og foreslår, at det kan være meget tilpasses andre organsystemer.

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Udviklingsmæssige biologi spørgsmålet 128 Konfokal mikroskopi billedanalyse kvantitative imaging organogenesis hjertet udvikling mus embryo
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