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Bewertung von In-vitro- DNA-Schäden mit Comet-Assay
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Bewertung von In-vitro- DNA-Schäden mit Comet-Assay

Article DOI: 10.3791/56450-v • 10:43 min • October 11th, 2017
October 11th, 2017 •
1,2, 1, 1
1Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, 2Basic Medical Science Department, Zunyi Medical College-Zhuhai Campus

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Die Comet-Assay ist eine effiziente Methode um zu erkennen, dass DNA-Schäden einschließlich Einzel- und Doppelstrang-DNA bricht. Wir beschreiben basische und neutrale Comet Assays zur Messung der DNA-Schäden in den Krebszellen, die therapeutische Wirkung der Chemotherapie zu bewerten.

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Zellbiologie Ausgabe 128 Chemo-Empfindlichkeit Gliom DNA-Schäden Comet-Assay einzelne Zelle Elektrophorese Assay Chemotherapie Genotoxizität Temozolomid Olaparib PARP
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