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Journal / Biochemistry / 化细胞穿透肽研究胞内蛋白-蛋白质相互作用…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
化细胞穿透肽研究胞内蛋白-蛋白质相互作用
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化细胞穿透肽研究胞内蛋白-蛋白质相互作用

Article DOI: 10.3791/56457-v • 10:26 min • December 20th, 2017
December 20th, 2017 •
1, 1,2, 1, 1, 1
1Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, 2Centre for Cancer Research & Cell Biology (CCRCB), School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast

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这是一项研究细胞内蛋白-蛋白质相互作用的协议, 基于生物素-素下拉系统, 具有新颖性的单元穿透序列结合。主要的优点是, 目标序列是用活细胞来培养的, 而不是细胞裂解, 因此相互作用将发生在细胞环境中。

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生物化学 问题 130 达 生物素素 细胞穿透肽 下拉 细胞内相互作用 蛋白质-蛋白质相互作用 西部印迹
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