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JoVE Journal Developmental Biology
Een RANKL gebaseerde Osteoclast cultuur Assay van muis beenmerg te onderzoeken van de rol van mTORC1 in Osteoclast vorming
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Een RANKL gebaseerde Osteoclast cultuur Assay van muis beenmerg te onderzoeken van de rol van mTORC1 in Osteoclast vorming

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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Dit manuscript wordt beschreven van een protocol bij isolaat en cultuur osteoclasten in vitro uit beenmerg van de muis, en te bestuderen van de rol van het zoogdier/mechanistische doelwit van rapamycin complexe 1 in osteoclast vorming.

Tags

Ontwikkelingsbiologie kwestie 133 Osteoclast mTORC1 Raptor bone omvorming celbiologie RANKL M-CSF
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