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Journal / Biochemistry / Proteome profunda de criação de perfil por…
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Biochemistry

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JoVE Journal Biochemistry
Proteome profunda de criação de perfil por isobárica rotulagem extensa cromatografia líquida, espectrometria de massa e quantificação assistida por Software
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Proteome profunda de criação de perfil por isobárica rotulagem extensa cromatografia líquida, espectrometria de massa e quantificação assistida por Software

Article DOI: 10.3791/56474-v • 10:37 min • November 15th, 2017
November 15th, 2017 •
1, 1, 1, 2,3, 1, 1, 2, 1,2
1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine

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Apresentamos um protocolo para dosar com precisão as proteínas com rotulagem isobárica, fracionamento extensivo, ferramentas de bioinformática e etapas de controle de qualidade em combinação com cromatografia líquida interfaceada com um espectrômetro de massa de alta resolução.

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Bioquímica edição 129 bioinformática interferência rotulagem isobárica cromatografia líquida de LC-MS/MS espectrometria de massa proteômica TMT
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