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Biochemistry

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JoVE Journal Biochemistry
Djupa proteomet profilering av isobariska märkning, omfattande vätskekromatografi, masspektrometri och programvara-assisted kvantifiering
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Djupa proteomet profilering av isobariska märkning, omfattande vätskekromatografi, masspektrometri och programvara-assisted kvantifiering

Article DOI: 10.3791/56474-v • 10:37 min • November 15th, 2017
November 15th, 2017 •
1, 1, 1, 2,3, 1, 1, 2, 1,2
1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine

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Vi presenterar ett protokoll för att exakt kvantifiera proteiner med isobariska märkning, omfattande fraktionering, bioinformatiska verktyg och kvalitetskontroll steg i kombination med vätskekromatografi kopplats ihop till en högupplöst masspektrometer.

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Biokemi frågan 129 bioinformatik störningar isobariska märkning LC-MS/MS flytande kromatografi masspektrometri proteomik TMT
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