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Preparazione del campione e la formazione immagine di esosomi da microscopia elettronica di trasmissione
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Preparazione del campione e la formazione immagine di esosomi da microscopia elettronica di trasmissione

Article DOI: 10.3791/56482-v • 11:15 min • January 4th, 2018
January 4th, 2018 •
1, 2
1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences, Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network, Korea Brain Research Institute

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Questo protocollo descrive le varie tecniche necessarie per microscopia elettronica di trasmissione tra cui macchiatura negativa, sezionamento ultrasottile per struttura dettagliata e immuno-oro etichettatura per determinare le posizioni di specifiche proteine in esosomi.

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Biologia cellulare numero 131 esosomi negativo macchiatura ultramicrotomia immunocolorazione microscopia elettronica a trasmissione vescicole extracellulari
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