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Forberedelse af prøver og billeddannelse af Exosomes af transmissions Elektron Mikroskopi
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Forberedelse af prøver og billeddannelse af Exosomes af transmissions Elektron Mikroskopi

Article DOI: 10.3791/56482-v • 11:15 min • January 4th, 2018
January 4th, 2018 •
1, 2
1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences, Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network, Korea Brain Research Institute

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Denne protokol beskriver de forskellige teknikker, der er nødvendige for transmissions Elektron Mikroskopi herunder negative farvning, ultratynde skæring for detaljerede struktur, og immuno-guld mærkning for at bestemme positioner af specifikke proteiner i exosomes.

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Cellebiologi sag 131 Exosome negative farvning ultramikrotomi immuno-farvning transmissions elektronmikroskopi ekstracellulære vesikler
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