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Bereiding van de monsters en de beeldvorming van de Exosomes door transmissie-elektronenmicroscopie
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Bereiding van de monsters en de beeldvorming van de Exosomes door transmissie-elektronenmicroscopie

Article DOI: 10.3791/56482-v • 11:15 min • January 4th, 2018
January 4th, 2018 •
1, 2
1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences, Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network, Korea Brain Research Institute

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Dit protocol beschrijft de verschillende technieken die nodig zijn voor de transmissie-elektronenmicroscopie, met inbegrip van negatieve kleuring, uiterst dunne segmenteren voor gedetailleerde structuur en immuno-goud etikettering om te bepalen van de standpunten van bepaalde eiwitten in exosomes.

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Celbiologie kwestie 131 Exosome negatieve kleuring ultramicrotomie immuno-kleuring transmissie-elektronenmicroscopie extracellulaire blaasjes
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