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Bereiding van de monsters en de beeldvorming van de Exosomes door transmissie-elektronenmicroscopie
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JoVE Journal Biology
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
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Bereiding van de monsters en de beeldvorming van de Exosomes door transmissie-elektronenmicroscopie

Nederlands

Automatically Generated

37,418 Views

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11:15 min

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January 04, 2018

DOI:

10.3791/56482-v

DOI:
10.3791/56482-v

•

11:15 min
January 04, 2018

•

39 Views
,
1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences,Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network,Korea Brain Research Institute

Summary

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Dit protocol beschrijft de verschillende technieken die nodig zijn voor de transmissie-elektronenmicroscopie, met inbegrip van negatieve kleuring, uiterst dunne segmenteren voor gedetailleerde structuur en immuno-goud etikettering om te bepalen van de standpunten van bepaalde eiwitten in exosomes.

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Cite this Article

Jung, M. K., Mun, J. Y. Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy. J. Vis. Exp. (131), e56482, doi:10.3791/56482 (2018).

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