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サンプル準備および透過電子顕微鏡によるエクソソームのイメージ投射
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JoVE Journal
Biology
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
サンプル準備および透過電子顕微鏡によるエクソソームのイメージ投射
DOI:
10.3791/56482-v
•
11:15 min
•
January 04, 2018
•
Min Kyo Jung
,
Ji Young Mun
1
Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences
,
Asan Medical Center
,
2
Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network
,
Korea Brain Research Institute
Chapters
00:05
Title
01:14
Exosome Block Preparation
03:38
Sectioning, Staining, and Imaging of the Exosome
04:27
Immuno-staining of Sections and Imaging
07:24
Whole Mount for Immunostaining
09:10
Results: Immunostained Exosome Sections Provide Insight to Their Protein Makeup
09:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルでは、否定的な汚損、詳細な構造と免疫金エクソソームの特定の蛋白質の位置を判断するための極薄断面透過型電子顕微鏡に必要な様々 なテクニックについて説明します。
Tags
Exosomes
Transmission Electron Microscopy
Sample Preparation
Imaging
Extracellular Vesicles
Protein Localization
Ultracentrifugation
Fixation
Dehydration
Embedding
Low Viscosity Embedding
Disease Diagnosis
Microcellular Incubation
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