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En primær menneskelige trofoblast Model til at studere effekten af Inflammation forbundet med maternel fedme på regulering af Autophagy i moderkagen
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En primær menneskelige trofoblast Model til at studere effekten af Inflammation forbundet med maternel fedme på regulering af Autophagy i moderkagen

Article DOI: 10.3791/56484-v • 11:44 min • September 27th, 2017
September 27th, 2017 •
*1, *2, 1
1Knight Cardiovascular Institute, Oregon Health and Science University, 2Department of Obstetrics and Gynecology, Oregon Health and Science University
* These authors contributed equally

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Præsenteres her er en protokol for prøvetagning af menneskelig placenta villøs væv efterfulgt af isolering af cytotrophoblasts for primære cellekultur. Behandling af trophoblasts med TNFα sammenfatter betændelse i fede intrauterin miljøet og letter opdagelsen af molekylære mål reguleret af betændelse i placenta med maternel fedme.

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Medicin sag 127 menneskelige placenta maternel fedme betændelse tumor nekrose faktor alfa (TNFα) villøs væv trophoblasts primære cellekultur vestlige skamplet autophagy Rubicon seksuelle dimorphism
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