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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation af diskriminerende menneskelige monoklonale antistoffer fra sjældne Antigen-specifikke B celler cirkulerer i blodet
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Generation af diskriminerende menneskelige monoklonale antistoffer fra sjældne Antigen-specifikke B celler cirkulerer i blodet

Article DOI: 10.3791/56508-v • 13:14 min • February 6th, 2018
February 6th, 2018 •
1,2,3, 1,2,3, 1,2, 1,2
1CRCINA, Inserm, CNRS, Université d'Angers, Université de Nantes, 2LabEx IGO, "Immunotherapy, Graft, Oncology", 3CHU Nantes, France

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Vi beskriver en metode til produktion af humane antistoffer specifikke for et antigen af interesse, med udgangspunkt i sjældne B celler cirkulerer i humant blod. Generation af disse naturlige antistoffer er effektiv og hurtig, og antistofferne fremstillet kan forskelsbehandle stærkt relaterede antigener.

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Immunologi sag 132 immunologi cellebiologi humane antistoffer B-celle tetramer flowcytometri enkelt celle antigen-specifikke magnetiske berigelse
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