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JoVE Journal Neuroscience
Overvågning af effekten af osmotisk Stress på sekretoriske vesikler og exocytose
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Overvågning af effekten af osmotisk Stress på sekretoriske vesikler og exocytose

Article DOI: 10.3791/56537-v • 08:08 min • February 19th, 2018
February 19th, 2018 •
1, 1, 2, 1
1Department of Chemistry and Chemical Engineering, Chalmers University of Technology, 2Department of Chemistry and Molecular Biology, University of Gothenburg

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Osmotisk stress påvirker exocytose og mængden af neurotransmitteren udgivet under denne proces. Vi demonstrere, hvordan kombinerer elektrokemiske metoder samt transmissions Elektron Mikroskopi kan bruges til at studere effekten af ekstracellulære osmotisk Tryk på exocytose aktivitet, vesikel quantal størrelse og mængden af neurotransmitteren frigivet under exocytose.

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Neurovidenskab sag 132 Chromaffin celler osmotisk tryk tæt kerne vesikler neurotransmitter koncentration quantal størrelse exocytose amperometry intracellulære flowcytometri transmissions elektronmikroskopi
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