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JoVE Journal Neuroscience
Analyse af immunceller i enkelt ischiadicus nerver og dorsalrods Ganglion fra et enkelt museklik bruge flowcytometri
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Analyse af immunceller i enkelt ischiadicus nerver og dorsalrods Ganglion fra et enkelt museklik bruge flowcytometri

Article DOI: 10.3791/56538-v • 12:34 min • December 6th, 2017
December 6th, 2017 •
1, 1,2,3, 1,3
1Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, 2Institute for Diabetes and Cancer IDC Helmholtz Center Munich, Germany & Joint Heidelberg-IDC Translational Diabetes Program, 3German Center for Diabetes Research (DZD)

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Kvantitativ analyse af celleindhold inden for murine iskiasnerven er vanskelig på grund af knaphed på vævet. Denne protokol beskriver en metode til væv fordøjelse og præparat, der giver tilstrækkelig celler flow flowcytometri analyse af immun cellepopulationer fra nerver af individuelle mus.

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Neurovidenskab spørgsmålet 130 flowcytometri mus iskiasnerven makrofag betændelse leukocytter neuropati
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