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March 28, 2018
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Ce protocole décrit une méthode pour l’imagerie simultanée de thalamocortical axon branching et synapse formation dans cocultures organotypique du thalamus et le cortex cérébral. Les axones thalamocortical individuels et leurs terminaisons présynaptiques sont visualisés par une technique d’électroporation monocellulaires avec DsRed et synaptophysine GFP-étiquetée.
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Cite this Article
Matsumoto, N., Yamamoto, N. Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures. J. Vis. Exp. (133), e56553, doi:10.3791/56553 (2018).
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