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Neuroscience

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JoVE Journal Neuroscience
Visualização do axônio Thalamocortical ramificação e sinapse formação em Organotypic Cocultures
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Visualização do axônio Thalamocortical ramificação e sinapse formação em Organotypic Cocultures

Article DOI: 10.3791/56553
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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March 28th, 2018

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Este protocolo descreve um método para tratamento de imagens simultâneo de thalamocortical axônio ramificação e sinapse formação em cocultures de organotypic do tálamo e córtex cerebral. Axônios de thalamocortical individuais e seus terminais pré-sináptica são visualizados pela técnica de eletroporação única célula com DsRed e Sinaptofisina GFP-etiquetado.

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