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Visualisation de Thalamocortical Axon Branching et Synapse Formation chez organotypique Cocultures
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Visualisation de Thalamocortical Axon Branching et Synapse Formation chez organotypique Cocultures

Article DOI: 10.3791/56553-v • 06:16 min • March 28th, 2018
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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Ce protocole décrit une méthode pour l’imagerie simultanée de thalamocortical axon branching et synapse formation dans cocultures organotypique du thalamus et le cortex cérébral. Les axones thalamocortical individuels et leurs terminaisons présynaptiques sont visualisés par une technique d’électroporation monocellulaires avec DsRed et synaptophysine GFP-étiquetée.

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Neurosciences numéro 133 tranche de culture cortex cérébral thalamus synaptogenèse arborisation axonale développement cortical imagerie Time-lapse
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