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连续切片电子断层扫描显示的 3D
果蝇
间接飞行肌的线粒体超微结构研究
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
3D Mitochondrial Ultrastructure of
Drosophila
Indirect Flight Muscle Revealed by Serial-section Electron Tomography
Please note that all translations are automatically generated.
Click here for the English version.
连续切片电子断层扫描显示的 3D
果蝇
间接飞行肌的线粒体超微结构研究
DOI:
10.3791/56567-v
•
06:45 min
•
December 19, 2017
•
Yi-fan Jiang
,
Hsiang-ling Lin
,
Chi-yu Fu
1
Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine
,
National Taiwan University
,
2
Institute of Cellular and Organismic Biology
,
Academia Sinica
Chapters
00:05
Title
00:37
Sectioning
Drosophila
Tissues with Vibrating Blade Microtome
01:19
High-pressure Freezing (HPF) and Freeze Substitution (FS) for Electron Microscope (EM)
02:30
Serial-sectioning for Electron Tomography
03:42
Double-title Electron Tomography
05:09
Results: Integrated Intra-mitochondria Cristae and Matrix Patterns in 3D
06:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
在本协议中, 我们展示了在
果蝇
间接飞行肌肉中应用的序列切片电子断层扫描来阐明线粒体结构。
Tags
3D
Mitochondrial Ultrastructure
Drosophila Indirect Flight Muscle
Serial-section Electron Tomography
Cellular Ultrastructure
High-pressure Freezing
Free Substitution
Resin Embedding
Ultramicrotomy
Transmission Electron Microscope
Dual-axis Tomography
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