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JoVE Journal Cancer Research
Se movendo para cima
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
Journal (Cancer Research)
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Journal (Cancer Research)
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
 
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Se movendo para cima: Um simples e flexível em Vitro invasão tridimensional protocolo do ensaio

Article DOI: 10.3791/56568-v • 06:14 min • March 12th, 2018
March 12th, 2018 •
1, 1,2,3,4,5, 6
1Department of Biomedical Engineering, University of Minnesota - Twin Cities, 2Stem Cell Institute, University of Minnesota - Twin Cities, 3Masonic Cancer Center, University of Minnesota - Twin Cities, 4Lillehei Heart Institute, University of Minnesota - Twin Cities, 5Institute for Engineering in Medicine, University of Minnesota - Twin Cities, 6Department of Biology, Jackson State University

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Este protocolo descreve um ensaio de invasão vertical invertida que poderia ser usado para quantificar os recursos de migração e invasão de células em uma configuração tridimensional, preservando o microambiente celular. Este ensaio é adequado para células raras e/ou sensíveis.

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Pesquisa sobre o câncer edição 133 invertido invasão migração tridimensional (3D) sensível microambiente raro
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