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Lente livre de microscopia para a dinâmica e a análise quantitativa de cultura de células aderentes
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Lente livre de microscopia para a dinâmica e a análise quantitativa de cultura de células aderentes

Article DOI: 10.3791/56580-v • 09:05 min • February 23rd, 2018
February 23rd, 2018 •
1, 1, 2, 2, 3,4, 3, 1, 1, 1, 5, 5, 4,6, 1
1CEA, LETI, DTBS, LISA, Université Grenoble Alpes, 2CEA, LETI, DTBS, LBAM, Université Grenoble Alpes, 3CEA, INSERM, BIG, Université Grenoble Alpes, 4CNRS, FR CNRS 3425, 5CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport, PSL Research University, Institut Curie, 6TIMC-IMAG

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Lente livre de microscopia nos permite monitorar culturas celulares diretamente dentro da incubadora. Aqui descrevemos o protocolo completo usado para adquirir e analisar uma aquisição de 2,7 dia longo de culturas de células de HeLa, levando a um conjunto de dados de 2,2 x 106 medições de morfologia de célula individual e 10584 ciclo celular rastreia.

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Biologia celular questão 132 livre de lente microscopia citometria de vídeo culturas de células densas microscopia quantitativa
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