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JoVE Journal
Biology

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无透镜视频显微术在黏附细胞培养动态定量分析中的研究
 
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无透镜视频显微术在黏附细胞培养动态定量分析中的研究

Article DOI: 10.3791/56580-v 09:05 min February 23rd, 2018
February 23rd, 2018

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Summary

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无透镜的视频显微镜使我们能够在孵化器内直接监测细胞培养。在这里, 我们描述了用于获取和分析2.7 天长时间获取培养的 HeLa 细胞的完整协议, 导致一个数据集 2.2 x 106测量单个细胞形态学和10584细胞周期轨道。

Tags

细胞生物学 问题 132 无透镜显微镜 视频细胞术 密集细胞培养 定量显微学
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