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Journal / Bioengineering / Detecção visual de vários ácidos nucleicos em uma …
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Bioengineering

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JoVE Journal Bioengineering
Detecção visual de vários ácidos nucleicos em uma matriz capilar
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Detecção visual de vários ácidos nucleicos em uma matriz capilar

Article DOI: 10.3791/56597-v • 08:56 min • November 15th, 2017
November 15th, 2017 •
*1,2,3, *1,2,3, *4, 4, 1,2,3, 4,5, 1,2,3, 6, 6, 4, 1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University
* These authors contributed equally

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Este protocolo descreve a fabricação de uma gaveta pequena, ready-to-use que pode ser aplicada para a detecção visual de vários ácidos nucleicos em um teste único, que é fácil de operar. Nesta abordagem, uma matriz capilar foi usado para multiplex e altamente eficiente detecção de alvos de OGM.

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Deteção de multiplex de ácido nucleico matriz lâmpada capilar bioengenharia edição 129 organismos (OGM) fácil de operar visual detecção geneticamente modificados
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