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Bioengineering

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JoVE Journal Bioengineering
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Visuell identifiering av flera Nucleic syror i en kapillär matris

Article DOI: 10.3791/56597-v • 08:56 min • November 15th, 2017
November 15th, 2017 •
*1,2,3, *1,2,3, *4, 4, 1,2,3, 4,5, 1,2,3, 6, 6, 4, 1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University
* These authors contributed equally

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Det här protokollet beskriver tillverkning av en liten, ready-to-use kassett som kan tillämpas för visuell identifiering av flera nucleic syror i en enda, test som är lätt att använda. I denna metod användes en kapillär matris för multiplex och högeffektiva påvisande av GMO mål.

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Bioteknik frågan 129 kapillär array lampa multiplex nukleinsyra upptäckt modifierade genetiskt organismer (GMO) lätt-to-operate visuell identifiering
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