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JoVE Journal Bioengineering
Visuele detectie van meerdere Nucleic Acids in een capillaire matrix
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Visuele detectie van meerdere Nucleic Acids in een capillaire matrix

Article DOI: 10.3791/56597-v • 08:56 min • November 15th, 2017
November 15th, 2017 •
*1,2,3, *1,2,3, *4, 4, 1,2,3, 4,5, 1,2,3, 6, 6, 4, 1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University
* These authors contributed equally

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Dit protocol beschrijft de fabricage van een kleine, kant-en-klare cassette die kan worden toegepast voor visuele detectie van meerdere nucleic zuren in een enkele, test die is eenvoudig te bedienen. In deze benadering werd een capillaire matrix gebruikt voor multiplex en zeer efficiënte opsporing van GGO-doelstellingen.

Tags

Bioengineering kwestie 129 capillair array LAMP multiplex nucleïnezuur detectie gemodificeerde genetisch organismen (GGO's) eenvoudig te bedienen visuele detectie
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