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Metode for mærkning udskrifter i individuelle Escherichia coli celler for enkelt-molekyle fluorescens In Situ hybridisering eksperimenter
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Metode for mærkning udskrifter i individuelle Escherichia coli celler for enkelt-molekyle fluorescens In Situ hybridisering eksperimenter

Article DOI: 10.3791/56600-v • 07:51 min • December 21st, 2017
December 21st, 2017 •
1, 1, 1
1Department of Physics of Complex Systems, Weizmann Institute of Science

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Denne manuskriptet beskrives en metode til mærkning individuelle messenger RNA (mRNA) udskrifter med fluorescently mærket DNA-sonder, til brug i enkelt-molekyle fluorescens i situ hybridisering (smFISH) eksperimenter i E. coli. smFISH er en visualisering metode, der giver mulighed for samtidige påvisning, lokalisering og kvantificering af enkelt mRNA molekyler i faste individuelle celler.

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Cellebiologi spørgsmålet 130 enkelt-molekyle fluorescens i situ hybridisering E. coli Gen-ekspression udskrift messenger RNA celle til celle variabilitet stokastiske optisk genopbygning mikroskopi
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