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Journal / Biology / 单分子荧光原位杂交实验中单个大肠埃希菌细胞的转录标记方法…
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单分子荧光原位杂交实验中单个大肠埃希菌细胞的转录标记方法
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单分子荧光原位杂交实验中单个大肠埃希菌细胞的转录标记方法

Article DOI: 10.3791/56600-v • 07:51 min • December 21st, 2017
December 21st, 2017 •
1, 1, 1
1Department of Physics of Complex Systems, Weizmann Institute of Science

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这篇手稿描述了一种方法, 标记个别信使 RNA (mRNA) 转录与荧光标记的 DNA 探针, 用于分子荧光原位杂交 (smFISH) 实验在大肠杆菌. smFISH 是一种可视化方法, 它允许在固定的单个细胞中同时检测、定位和量化单个 mRNA 分子。

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细胞生物学 问题 130 单分子荧光原位杂交,大肠杆菌 基因表达 转录 信使 RNA 细胞变异性 随机光学重建显微镜
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