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Metode for merking utskrifter i individuelle Escherichia coli celler for Single-molekylet fluorescens In Situ hybridisering eksperimenter
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Metode for merking utskrifter i individuelle Escherichia coli celler for Single-molekylet fluorescens In Situ hybridisering eksperimenter

Article DOI: 10.3791/56600-v • 07:51 min • December 21st, 2017
December 21st, 2017 •
1, 1, 1
1Department of Physics of Complex Systems, Weizmann Institute of Science

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Dette manuskriptet beskriver en metode for merking personlige budbringer RNA (mRNA) utskrifter med fluorescently-merket DNA sonder, for bruk i enkelt-molekylet fluorescens i situ hybridisering (smFISH) eksperimenter i E. coli. smFISH er en visualisering metode som tillater samtidig deteksjon, lokalisering og kvantifisering av enkelt mRNA molekyler i fast enkeltceller.

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Mobilnettet biologi problemet 130 Single-molekylet fluorescens i situ hybridisering E. coli genekspresjon transkripsjon budbringer RNA celle-til-celle variasjon stokastiske optisk rekonstruksjon mikroskopi
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