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Journal / Developmental Biology / Einzellige Quantifizierung der Protein-Abbauraten …
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Einzellige Quantifizierung der Protein-Abbauraten von Time-Lapse Fluoreszenzmikroskopie in adhärente Zellkultur
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Einzellige Quantifizierung der Protein-Abbauraten von Time-Lapse Fluoreszenzmikroskopie in adhärente Zellkultur

Article DOI: 10.3791/56604-v • 12:07 min • February 4th, 2018
February 4th, 2018 •
1, 1
1UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)

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Dieses Protokoll beschreibt eine Methode zum Bestimmen von Protein in einzelne lebende adhärente Zellen, Halbwertszeiten mit Puls Kennzeichnung und Zeitraffer Fluoreszenz-Bildgebung von Fusionsproteinen SNAP-Tag.

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Entwicklungsbiologie Ausgabe 132 Proteinabbau SNAP-Tag einzelne Zellen adhärente Zellkultur embryonalen Stammzellen der Maus Fluoreszenz-Mikroskopie live Cell Imaging Kennzeichnung fluoreszierenden Farbstoff Puls
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