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JoVE Journal
Cancer Research

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Multipleksede immunofluorescens analyse og kvantificering af Intratumoral PD-1+ Tim-3+ CD8+ T celler
 
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Multipleksede immunofluorescens analyse og kvantificering af Intratumoral PD-1+ Tim-3+ CD8+ T celler

Article DOI: 10.3791/56606-v 09:32 min February 8th, 2018
February 8th, 2018

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Summary

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At studere tumor mikromiljø kan identificere prognostiske eller intelligent biomarkører for klinisk respons på immunterapi. Præsenteres her, er en nyskabende metode baseret på i situ fluorescens multispektrale imaging for at analysere og regne automatisk forskellige delpopulationer af CD8+ T celler. Denne reproducerbare og pålidelige teknik egner sig til stor kohorte analyser.

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Immunologi sag 132 Multiplex i situ multispektrale imaging immunofluorescens teknik digital billedbehandling renalcellecarcinom Tim-3 PD-1 tumor mikromiljø checkpoint hæmmere i situ enkelt celle analyse
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