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Cancer Research

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JoVE Journal Cancer Research
Multiplexado imunofluorescência análise e quantificação de Intratumoral PD-1+ + Tim-3 CD8+ T células
Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs
Journal (Cancer Research)
Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs
Capture and Release of Viable Circulating Tumor Cells from Blood
Journal (Cancer Research)
Capture and Release of Viable Circulating Tumor Cells from Blood
 
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Multiplexado imunofluorescência análise e quantificação de Intratumoral PD-1+ + Tim-3 CD8+ T células

Article DOI: 10.3791/56606-v • 09:32 min • February 8th, 2018
February 8th, 2018 •
1, 2,3,4, 2,3, 2,3, 2,3,5, 6, 7, 2, 2, 2, 2,3,6, 8, 2,3, 4, 2,6, *4, *2,4
1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou
* These authors contributed equally

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Estudar o microambiente do tumor pode identificar biomarcadores prognósticos ou preditivos de resposta clínica à imunoterapia. Apresentado aqui, é um método inovador baseado em situ fluorescência multiespectral de imagens para analisar e contar automaticamente várias subpopulações de CD8+ T células. Esta técnica reprodutível e confiável é apropriada para análises de grandes coorte.

Tags

Imunologia questão 132 Multiplex em situ imagens multiespectrais técnica de imunofluorescência imagem digital carcinoma de células renais Tim-3 o PD-1 microambiente do tumor inibidores de ponto de verificação análise de célula única em situ
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