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Journal / Cancer Research / Multiplexed immunofluorescens analys och…
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Cancer Research

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JoVE Journal Cancer Research
Multiplexed immunofluorescens analys och kvantifiering av Intratumoral PD-1+ Tim-3+ CD8+ T celler
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Multiplexed immunofluorescens analys och kvantifiering av Intratumoral PD-1+ Tim-3+ CD8+ T celler

Article DOI: 10.3791/56606-v • 09:32 min • February 8th, 2018
February 8th, 2018 •
1, 2,3,4, 2,3, 2,3, 2,3,5, 6, 7, 2, 2, 2, 2,3,6, 8, 2,3, 4, 2,6, *4, *2,4
1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou
* These authors contributed equally

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Studera tumören närmiljön kan identifiera prediktiva eller prognostiska biomarkörer för kliniskt svar på immunterapi. Presenteras här, är en innovativ metod baserad på i situ fluorescens Multispektral avbildning för att analysera och räkna automatiskt olika subpopulationer av CD8+ T celler. Denna reproducerbara och tillförlitlig teknik är lämplig för stor kohort analyser.

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Immunologi fråga 132 Multiplex i situ Multispektral avbildning immunofluorescens-teknik digital bildbehandling njurcellscancer Tim-3 PD-1 tumör mikromiljö checkpoint-hämmare i situ enda cell analys
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