Journal
/
Genetics
/
Konstituerende og inducerbar systemer for genetiske In Vivo ændring af mus hepatocytter brug af hydrodynamiske hale vene injektion
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Konstituerende og inducerbar systemer for genetiske In Vivo ændring af mus hepatocytter brug af hydrodynamiske hale vene injektion

English

Automatically Generated

13,711 Views

•

09:35 min

•

February 02, 2018

DOI:

10.3791/56613-v

DOI:
10.3791/56613-v

•

09:35 min
February 02, 2018

•

16 Views
, , , , ,
1Department of Medicine II, Klinikum rechts der Isar,Technische Universität München, 2Department of Pneumology, Center for Medicine,Medical Center University of Freiburg

Summary

Automatically generated

Hydrodynamisk hale vene injektion af transposon-baseret integration vektorer giver stabil Transfektion af murine hepatocytter in vivo. Vi præsenterer her, en praktisk protokol til Transfektion systemer, der giver mulighed for den langsigtede konstitutiv udtryk for en enkelt transgen eller kombinerede konstitutiv og doxycyclin-inducerbar udtryk for en transgen eller miR-shRNA i leveren.

Explore More Videos

In VivoGenetic ModificationMouse HepatocytesHydrodynamic Tail Vein InjectionConstitutive SystemInducible SystemCreERShRNAVector ConstructLiver ResearchLiver CancerLiver Regeneration

Read Article

Cite this Article

Hubner, E. K., Lechler, C., Rösner, T. N., Kohnke-Ertel, B., Schmid, R. M., Ehmer, U. Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection. J. Vis. Exp. (132), e56613, doi:10.3791/56613 (2018).

Download .ris file

Copy

Share Video

.

Copy