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JoVE Journal Genetics
Konstituerende og inducerbar systemer for genetiske In Vivo ændring af mus hepatocytter brug af hydrodynamiske hale vene injektion
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Konstituerende og inducerbar systemer for genetiske In Vivo ændring af mus hepatocytter brug af hydrodynamiske hale vene injektion

Article DOI: 10.3791/56613-v • 09:35 min • February 2nd, 2018
February 2nd, 2018 •
1,2, 1, 1, 1, 1, 1
1Department of Medicine II, Klinikum rechts der Isar, Technische Universität München, 2Department of Pneumology, Center for Medicine, Medical Center University of Freiburg

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Hydrodynamisk hale vene injektion af transposon-baseret integration vektorer giver stabil Transfektion af murine hepatocytter in vivo. Vi præsenterer her, en praktisk protokol til Transfektion systemer, der giver mulighed for den langsigtede konstitutiv udtryk for en enkelt transgen eller kombinerede konstitutiv og doxycyclin-inducerbar udtryk for en transgen eller miR-shRNA i leveren.

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Genetik sag 132 leveren transposon systemer Tornerose Tet-on hydrodynamiske hale vene injektion i vivo Transfektion video guide.
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