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Journal / Bioengineering / 改良的3D 水凝胶培养的原发胶质细胞的体外Neuroinflammation…
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Bioengineering

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JoVE Journal Bioengineering
改良的3D 水凝胶培养的原发胶质细胞的体外Neuroinflammation 模型的建立
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改良的3D 水凝胶培养的原发胶质细胞的体外Neuroinflammation 模型的建立

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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在此, 我们为大鼠脑源性胶质细胞的3D 培养提供了一项协议, 包括星状细胞、小胶质和突。我们展示了原细胞培养, methacrylated 透明质酸 (哈马) 水凝胶合成, HAMAphoto 聚合和细胞封装, 和样品处理共聚焦和扫描电子显微成像。

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生物工程 问题 130 3D 细胞培养 水凝胶 原胶质 星形胶质 小胶质 胶质 neuroinflammation 透明质酸 哈马
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