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JoVE Journal Bioengineering
3D Hydrogel Kulturen von primären Gliazellen für In-vitro- Modellierung von Neuroinflammation verbessert
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3D Hydrogel Kulturen von primären Gliazellen für In-vitro- Modellierung von Neuroinflammation verbessert

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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Hier präsentieren wir ein Protokoll für die 3D Kultur der Ratte Gehirn abgeleitet Glia-Zellen, einschließlich der Oligodendrozyten, Astrozyten und Mikroglia. Wir zeigen Primärzelle Kultur, methacrylated Hyaluronsäure (HAMA) Hydrogel Synthese, HAMAphoto-Polymerisation und Zelle Kapselung und Probe Verarbeitung für die konfokale und scanning Electron mikroskopische Bildgebung.

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Biotechnik Ausgabe 130 3D Zellkultur Hydrogel primären Gliazellen Astrozyten Mikroglia Oligodendrozyt Neuroinflammation Hyaluronsäure HAMA
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