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Journal / Bioengineering / Forbedret 3D Hydrogel kulturer av primære…
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JoVE Journal Bioengineering
Forbedret 3D Hydrogel kulturer av primære gliacellene for In Vitro modellering av Neuroinflammation
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Forbedret 3D Hydrogel kulturer av primære gliacellene for In Vitro modellering av Neuroinflammation

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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Her presenterer vi en protokoll for 3D kultur rotte hjerne-avledet glia cellene, inkludert astrocyttene, microglia og oligodendrocytes. Viser vi primære cellekultur, methacrylated hyaluronsyre (HAMA) hydrogel syntese, HAMAphoto-polymerisasjon og celle innkapsling og eksempel behandling for AC confocal og skanning elektron mikroskopiske bildebehandling.

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Bioteknologi problemet 130 3D cellekultur hydrogel primære glia astrocytter microglia oligodendrocyte neuroinflammation hyaluronsyre HAMA
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