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Journal / Biology / Fleksibel måling af en bioluminescerende…
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JoVE Journal Biology
Fleksibel måling af en bioluminescerende journalister ved hjælp af en automatiseret langsgående Luciferase Imaging Gas - og temperatur-optimeret Recorder (ALLIGATOR)
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Fleksibel måling af en bioluminescerende journalister ved hjælp af en automatiseret langsgående Luciferase Imaging Gas - og temperatur-optimeret Recorder (ALLIGATOR)

Article DOI: 10.3791/56623-v • 10:33 min • December 13th, 2017
December 13th, 2017 •
1, 1, 1
1MRC Laboratory of Molecular Biology

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Genetisk kodet luciferase er en populær non-invasiv reporter af genekspression. Anvendelse af en automatiseret langsgående luciferase imaging gas - og temperatur-optimeret recorder (ALLIGATOR) gør det muligt for langsgående optagelse fra en bioluminescerende celler under en lang række betingelser. Her viser vi, hvordan ALLIGATOR kan anvendes i forbindelse med døgnrytmen forskning.

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Molekylærbiologi spørgsmålet 130 en bioluminescerende reportere døgnrytmen ALLIGATOR PERIOD2 luciferase perfusion
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