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In Vitro Ensaio de SUMOylation para estudar a atividade de Ligase E3 sumô
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In Vitro Ensaio de SUMOylation para estudar a atividade de Ligase E3 sumô

Article DOI: 10.3791/56629-v • 09:45 min • January 29th, 2018
January 29th, 2018 •
1, 2, 2,3,4,5, 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

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Ao contrário da ubiquitina ligases, foram identificados alguns ligases E3 sumô. Este modificado em vitro protocolo SUMOylation é capaz de identificar o romance sumô E3 ligases por um ensaio de reconstituição em vitro .

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Biologia molecular questão 131 SUMOylation In vitro K-bZIP sumô E3 ligase p53 modificação pós-traducional Ubc9
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