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JoVE Journal Neuroscience
Isolamento e coltura dei progenitori neurali seguito da immunoprecipitazione della cromatina di istone 3 lisina 79 Dimethylation Mark
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Isolamento e coltura dei progenitori neurali seguito da immunoprecipitazione della cromatina di istone 3 lisina 79 Dimethylation Mark

Article DOI: 10.3791/56631-v • 10:09 min • January 26th, 2018
January 26th, 2018 •
1,2, 1, 1, 1, 1
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

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Presentiamo un metodo efficace e riproducibile per isolare e coltura di cellule progenitrici neurali dal tessuto di cervello embrionale e postnatale per immunoprecipitazione della cromatina (ChIP) di istone 3 lisina 79 dimethylation (H3K79me2) - un contrassegno dell'istone si trova all'interno del dominio globulare dell'istone 3.

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Neuroscienze problema 131 coltura delle cellule cerebellari coltura delle cellule corticali sviluppo di corteccia cerebellare H3K79 metilazione DOT1L ChIP inibizione di DOT1L
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