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Journal / Neuroscience / 组蛋白3赖氨酸 79 Dimethylation…
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JoVE Journal Neuroscience
组蛋白3赖氨酸 79 Dimethylation 标记沉淀后神经祖细胞的分离培养
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组蛋白3赖氨酸 79 Dimethylation 标记沉淀后神经祖细胞的分离培养

Article DOI: 10.3791/56631-v • 10:09 min • January 26th, 2018
January 26th, 2018 •
1,2, 1, 1, 1, 1
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

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我们提出一种有效和可重复的方法, 分离和培养胚胎和产后脑组织中的神经祖细胞染色质沉淀 (芯片) 的组蛋白3赖氨酸 79 dimethylation (H3K79me2)-一个组蛋白标记位于组蛋白3的球状域。

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神经科学 问题 131 小脑细胞培养 皮层细胞培养 皮质发育 小脑发育 H3K79 甲基化 DOT1L 芯片 DOT1L 抑制
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